prior_to

Reaction events (or reaction steps) can occur step by step. This relation can indicate the temporal order of two events.

active site, where a bound water is for nucleophilic attack.
T1 EntityProperty 0 11 active site
T2 Interaction 21 26 bound
T3 OtherCompound 27 32 water
T4 NucleophilicAttack 40 59 nucleophilic attack
E1 Interaction:T2 Theme:T1 Theme2:T3
E2 NucleophilicAttack:T4 Agent:T3
R1 prior_to Arg1:E1 Arg2:E2

Some words/phrases, such as “after”, “before” and “result in”, may suggest the temporal order of events.

Cys-SOH is formed after attack of the thiolate on H2O2.
T1 OtherCompound 0 7 Cys-SOH
T2 AminoAcid 0 3 Cys
T3 FunctionalGroup 4 7 SOH
T4 BondFormation 11 17 formed
T5 NucleophilicAttack 24 30 attack
T6 FunctionalGroup 38 46 thiolate
T7 OtherCompound 50 54 H2O2
E1 BondFormation:T4 Theme:T1
E2 NucleophilicAttack:T5 Agent:T6 Theme:T7
R1 whole_part Arg1:T1 Arg2:T2
R2 whole_part Arg1:T1 Arg2:T3
R3 prior_to Arg1:E2 Arg2:E1

A nucleophilic attack at the sugar C-1' of the nucleoside results in scission of the N-glycosylic bond.
T1 NucleophilicAttack 2 21 nucleophilic attack
T2 FunctionalGroup 29 38 sugar C-1'
T3 FunctionalGroup 47 57 nucleoside
T4 Cleavage 69 77 scission
T5 FunctionalGroup 85 102 N-glycosylic bond
E1 NucleophilicAttack:T1 Theme:T2
E2 Cleavage:T4 Theme:T5
R1 whole_part Arg1:T3 Arg2:T2
R2 prior_to Arg1:E1 Arg2:E2

Asp102 stabilizes the charged His57, and orients the conformation of His57 correctly. His57 deprotonates the hydroxyl group of Ser195, which makes a nucleophilic attack on the carbonyl carbon atom of the scissile bond, peptide bond, leading to the formation of a tetrahedral acyl-intermediate.
T1 AminoAcid 0 6 Asp102
T2 Stabilisation 7 17 stabilizes
T3 Activation 22 29 charged
T4 AminoAcid 30 35 His57
T5 Modulation 41 48;53 65 orients conformation
T6 AminoAcid 69 74 His57
T7 AminoAcid 86 91 His57
T8 Deprotonation 92 104 deprotonates
T9 FunctionalGroup 109 123 hydroxyl group
T10 AminoAcid 127 133 Ser195
T11 NucleophilicAttack 141 146;149 168 makes nucleophilic attack
T12 FunctionalGroup 176 196 carbonyl carbon atom
T13 EntityProperty 204 217 scissile bond
T14 FunctionalGroup 219 231 peptide bond
T15 BondFormation 248 257 formation
T16 EntityProperty 263 292 tetrahedral acyl-intermediate
E1 Stabilisation:T2 Agent:T1 Theme:T4
E2 Activation:T3 Theme:T4
E3 Modulation:T5 Agent:T1 Theme:T6
E4 Deprotonation:T8 Agent:T7 Theme:T9
E5 NucleophilicAttack:T11 Agent:T9 Theme:T12
E6 BondFormation:T15 EndPoint:T16 Agent:T9 Agent2:T12
R1 whole_part Arg1:T10 Arg2:T9
R2 whole_part Arg1:T14 Arg2:T12
R3 corresponds_to Arg1:T13 Arg2:T14
* simultaneous E1 E2 E3
R4 prior_to Arg1:E3 Arg2:E4
R5 prior_to Arg1:E4 Arg2:E5
R6 prior_to Arg1:E5 Arg2:E6
A1 EntityAttribute T13 ReactivePart
A2 EntityAttribute T16 ReactionState